Data Sheet 1_Ginsenoside Rb2 promotes p62-mediated LDHA autophagic degradation to inhibit H3K18la-dependent PD-L1 transcription in cervical cancer.pdf

This study investigated the effects of Rb2 on cervical cancer progression and explored whether metabolic reprogramming contributes to PD-L1 regulation.</p>Methods<p>Cell proliferation, migration, invasion, glycolytic activity, lactate production, histone lactylation, and PD-L1 expression were evaluated in cervical cancer cells using CCK-8, EdU, colony formation, wound-healing, Transwell, Seahorse analysis, RT-qPCR, western blotting, flow cytometry, and ChIP-qPCR.

LDHA regulation was examined by protein and mRNA stability assays, pharmacological inhibition, lysosome immunoprecipitation, gain- and loss-of-function studies, co-immunoprecipitation, molecular docking, and biotinylated Rb2 pull-down assays. T-cell function was assessed by Jurkat T-cell–tumor cell co-culture and IL-2 ELISA. Xenograft models and clinical samples were used for validation.</p>Results<p>Rb2 significantly inhibited cervical cancer cell proliferation, migration, invasion, and xenograft tumor growth, reducing HeLa and SiHa cell viability by approximately 23.4% and 25.7%, respectively.