Data · dataset · 2023
In vivo two-photon calcium imaging dataset obtained from the primary visual cortex of GCaMP6 mice during electrical microstimulation
Listed in ARP Research Data Repository
Here, we deposited a calcium imaging dataset collected within the framework of the HYPERSTIM Horizon Europe project.
Description
The in vivo dataset was obtained from the primary visual cortex of GCaMP6 transgenic mice during electrical microstimulation using a two-photon laser scanning microscope. A subset of the dataset contains spontaneous cortical activity without electrical stimulation (Mouse1 and Mouse2).
Mice were anesthetized with ketamine/xylazine. A Femtonics Femto2D two-photon microscope setup was used for data acquisition. A 20x water immersion objective with the following parameters was used for calcium imaging.
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Field of view (FOV): 550 μm x 550 μm; Numerical aperture (NA): 1.0; Working distance: 2.0 mm. We performed raster scanning with a resonant scanner at ~31 Hz frame rate and with a frame size of 512 x 512 pixels. The wavelength used for calcium imaging was 900 nm.
Further details can be found below (see Notes).
Links
Where it is published
- Dataverse dataset page repo.researchdata.hu/dataset.xhtml?persistentId=hdl%3A21.15109%2FCONCORDA%2FITBQD4 ↗
landing page · from repo researchdata hu
- Persistent identifier hdl.handle.net/21.15109/CONCORDA/ITBQD4 ↗
DOI / persistent id · from repo researchdata hu
Catalogue records · 1
- Dataverse API repo.researchdata.hu/api/datasets/:persistentId/?persistentId=hdl%3A21.15109%2FCONC… ↗
metadata API · from repo researchdata hu
Topics
- Stated by source
- Medicine, Health and Life Sciences
- From keywords
- Engineering · Life Sciences · Medicine & Health
- Inferred from text
- Biochemistry and cell biology 71% · Imaging 75%
Provenance · 1 source records, 12 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| ARP Research Data Repository | hdl:21.15109/CONCORDA/ITBQD4 | 13 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| concepts[field].anzsrc:group:3101 | enrichment · repo researchdata hu | taxonomy-embedding@1.1.0 | title+keywords+description (71%) |
| concepts[field].dataverse_subject:medicine-health-and-life-sciences | source · repo researchdata hu | connector:repo_researchdata_hu@1.0.0 | /subjects |
| concepts[field].local:field:engineering | mapping · repo researchdata hu | connector:repo_researchdata_hu@1.0.0 | /subjects |
| concepts[field].local:field:life-sciences | mapping · repo researchdata hu | connector:repo_researchdata_hu@1.0.0 | /subjects |
| concepts[field].local:field:medicine-health | mapping · repo researchdata hu | connector:repo_researchdata_hu@1.0.0 | /subjects |
| concepts[modality].local:modality:imaging | enrichment · repo researchdata hu | keyword-concept-rules@1.0.0 | title+description (75%) |
| created_date | source · repo researchdata hu | connector:repo_researchdata_hu@1.0.0 | |
| description | source · repo researchdata hu | connector:repo_researchdata_hu@1.0.0 | /description |
| publication_date | source · repo researchdata hu | connector:repo_researchdata_hu@1.0.0 | |
| title | source · repo researchdata hu | connector:repo_researchdata_hu@1.0.0 | /name |
| updated_date | source · repo researchdata hu | connector:repo_researchdata_hu@1.0.0 | |
| version_label | source · repo researchdata hu | connector:repo_researchdata_hu@1.0.0 |