<p>Analysis of nuclear metabolic enzymes across the <i>T. cruzi</i> cell cycle.</p>

<p>Proportion of nuclear proteins associated with metabolism (5.96%) identified using GO terms related to key metabolic processes, including “cellular amino acid metabolic process” (GO:0006520), “carbohydrate metabolic process” (GO:0005975), “pyruvate metabolic process” (GO:0006090), “ATP metabolic process” (GO:00046034), “nucleotide metabolic process” (GO:0009116), “nucleoside metabolic process” (GO:0009117), “lipid metabolic process” (GO:0006629), and “generation of precursor metabolites and energy” (GO:0006091). <b>(B)</b> KEGG pathway enrichment analysis of the metabolic enzymes identified in <b>(A)</b>, performed using TriTrypDB’s tool with a Bonferroni-corrected p-value cutoff of 0.05.

Enriched pathways are shown according to fold enrichment. <b>(C)</b> Venn diagram comparing the nuclear proteins identified in this study (CC_PN) with those present in the nuclear proteome (CC_PT) (De Castro Moreira Dos Santos et al., 2015) and those at chromatin proteome (Chr_P) (De Jesus et al., 2017). <b>(D)</b> Hierarchical clustering analysis of all protein expression profiles across different cell cycle phases. The protein data abundance was normalized before clustering by the Euclidean distance ratio. <b>(E)</b> Venn diagram between the nuclear cell cycle proteins identified in this study (CC_NP) and the Cell cycle proteome (CC_P) (Santos Júnior et al., 2021). <b>(F)</b> Box plots representing the relative abundance (%) of nuclear metabolic proteins found in (A) and ribosomal proteins across cell cycle phases.</p>