Data · dataset · 2025
Original Image of Guzhi Zengsheng Zhitong Decoction Mitigates Osteoarthritis via FOXO1-Mediated Autophagy and Apoptosis
Listed in Teesside University Research Data Repository
Description
figure 1 (B-D) Representative histological images of knee joints after 4 weeks of treating, including hematoxylin-eosin (HE), safranin-O fast green, and toluidine blue staining. figure 2 (A) Micro-CT images showing 3D reconstructions of the distal femur and transverse and sagittal views of the tibial plateau. figure 6 (I, J) Immunohistochemical analysis and quantification of FoxO1, LC3B, Parkin and PINK1 expression in the rat articular cartilage following oral administration (n=4, scale bar: 200 μm). figure 7 (A) Representative transmission electron microscopy images showing mitochondrial morphology (scale bars: 5 μm, 2 μm, 500 nm).
(C) Fluorescence images of mitochondrial membrane potential (MMP) staining. Red fluorescence represents JC-1 aggregates, green fluorescence represents JC-1 monomers, and the red/green intensity ratio reflects MMP (scale bar: 100 μm). (D) Fluorescence images of mitochondrial permeability transition pore (MPTP) staining (scale bar: 200 μm).
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(E) Fluorescence images of reactive oxygen species (ROS) levels (scale bar: 200 μm). figure 8 (C) TUNEL staining to evaluate apoptosis in knee cartilage of OA rats after oral GZZD treatment. (scale bar: 100 μm). figure 10 (A) JC-1 staining showing mitochondrial membrane potential (MMP) in OA chondrocytes with and without FoxO1 silencing (scale bar: 200 μm). (C) Effects of FoxO1 silencing on mitochondrial MPTP permeability after GZZD treatment.
(scale bar: 200 μm). (E) ROS levels visualized by fluorescence staining before and after FoxO1 silencing. (scale bar: 200 μm).
Supplement figures Figure S1. Representative anatomical images of rat knee joints at week 4 post-treatment. Figure S2.
Identification of primary chondrocytes. (A) Observation of cell morphology; (B) COL2A1 immunofluorescence staining. Figure S5.
Effects of GZZD on OA chondrocyte apoptosis observed via Annexin V-FITC/PI staining (in vitro). Figure S8. Effects of oral GZZD on STAT3, FOXO1, P-P65, and P65 protein levels in OA rat chondrocytes analyzed by Western blot and quantitative analysis, in vivo Western Blot Original Image.
Links
Where it is published
- DOI doi.org/10.17632/kd7n8xdr3t.1 ↗
DOI / persistent id · from researchdata tees ac uk
Catalogue records · 1
- OAI-PMH record data.mendeley.com/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=oai%3Adata… ↗
metadata API · from researchdata tees ac uk
Topics
- From keywords
- Earth & Environmental Science · Engineering · Humanities · Life Sciences · Social Science
- Inferred from text
- Image 75% · Microscopy 75%
Provenance · 1 source records, 12 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| Teesside University Research Data Repository | oai:data.mendeley.com/kd7n8xdr3t.1 | 9 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | |
| concepts[field].local:field:engineering | mapping · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | |
| concepts[field].local:field:humanities | mapping · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | |
| concepts[field].local:field:social-science | mapping · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | |
| concepts[modality].local:modality:image | enrichment · researchdata tees ac uk | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:microscopy | enrichment · researchdata tees ac uk | keyword-concept-rules@1.0.0 | title+description (75%) |
| description | source · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | /metadata/dc/description |
| license | source · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | /metadata/dc/rights |
| publication_date | source · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | |
| title | source · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | /metadata/dc/title |