Excel · study · 2026
Suppression of upstream ORF translation is not the primary mechanism for translational stimulation by yeast helicase Ded1
Listed in NCBI GEO
Impairment of DEAD-box RNA helicase Ded1 function results in substantial reduction in translational efficiencies (TEs) of main open reading frames (mORFs) in budding yeast.
Description
However, it was unknown whether this translation reduction in ded1 mutants is brought about by the increased translation of upstream open reading frames (uORFs).To examine this, we combined ribosome footprint profiling with RNA-seq analysis of mRNA abundance and determined the relative changes in TEs of uORFs and mORFs in ded1 mutants versus wild-type cells.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE311nnn/GSE311042 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE311042 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1368593 ncbi.nlm.nih.gov/bioproject/PRJNA1368593 ↗
project · from NCBI GEO
- PubMed 42087789 pubmed.ncbi.nlm.nih.gov/42087789 ↗
publication · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Saccharomyces cerevisiae
- From keywords
- Life Sciences
- Inferred from text
- Biochemistry and cell biology 70% · RNA sequencing 65%
Provenance · 1 source records, 9 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE311042 | 15 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].anzsrc:group:3101 | enrichment · NCBI GEO | taxonomy-embedding@1.1.0 | title+keywords+description (70%) |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (65%) |
| concepts[organism].NCBITaxon:4932 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |