Circulating Plasma MicroRNA Expression in Early-onset and Late-onset Coronary Artery Disease Patients

To improve CAD diagnosis, microRNAs are being explored as potential minimally invasive biomarkers. This dataset contains processed and analysis-ready data from a study of circulating plasma microRNAs (miRNAs) in patients with early-onset and late-onset coronary artery disease (CAD) and controls (29 controls, 80 early-onset CAD, 28 late-onset CAD).

EDTA plasma samples were collected prior to coronary angiography, total RNA enriched for small RNAs was isolated, and small RNA libraries were prepared and sequenced on an Illumina platform; primary preprocessing included quality control (FastQC), adapter trimming and miRNA quantification with miRDeep2, alignment to the GRCh38.p13 reference genome with Bowtie, and annotation against miRBase v22.1, followed by filtering of lowly expressed miRNAs, batch-effect correction with ComBat-seq, TMM normalisation, and differential expression analysis with limma and empirical Bayes moderation.