RNA binding protein YWHAZ mediates translation of specific mRNA and regulates cell Proliferation and apoptosis in diabetic foot ulcer

Cellular experiments revealed that Si_YWHAZ facilitated proliferation and migration while inhibiting apoptosis, consistent with its upregulation in DFU. RNA-seq identified 1072 DEGs in Si_YWHAZ cells.

Up-regulated genes were significantly enriched in cell proliferation-related processes and included AREG, FOSL1, HAS2 and IL7R, whereas down-regulated genes were associated with cell adhesion, such as LAMB3, SLAMF7, COL12A1 and ITGA5. IRIP-seq results demonstrated that YWHAZ interacts with plentiful of mRNAs and genomic location toward the CD and introns region. As well, ABLIFE algorithm indicated that YWHAZ bound to GC-rich motifs.