R script and replication data for: A simple and practical method to increase the infectivity of wild isolates of Plasmodium falciparum to mosquito vectors

Hence, patent adaptations to permanent irrigated schemes, urban settlements and wide insecticides use lead to the conspicuous extension of the repartition area of Anopheles species, and set the scene for the emergence or re-emergence of sustained malaria transmission.

Physiological mechanisms allowing malaria mosquitoes to colonize new human-made ecological niches are under scrutiny, however, less attention is given to behavioral modifications triggered by -or in response to-relevant environmental cues characterizing the new ecological niches. A simple, field applicable method is presented for improving parasite infectivity by concentrating Plasmodium falciparum gametocytes. Anopheles gambiae received one of the following 5 blood treatments through DMFA: (i) whole blood (WB) samples from naturally-infected donors; (ii) donor blood whose plasma was replaced with the same volume of malaria-naive AB+ serum (1:1 control); (iii) plasma replaced with a volume of malaria-naïve AB+ serum equivalent to half (2:1), or to a quarter (4:1), of the initial plasma volume; and (v) donor blood whose plasma was fully removed (RBC).