Identification, isolation and bioactivity assessment of a bioactive peptide QUB-1465 from the skin secretion of Hylarana guentheri

Among these natural peptides, AMPs have attracted much attention because of its potential to replace traditional antibiotics and solve the problem of microbial resistance as AMPs kill microorganisms through a membrane degradation mechanism, as described in the “barrel-stave-pore model”, the “toroidal-pore model” and the “carpet-like model”.

In this research, a single cDNA sequence encoding a bioactive peptide QUB-1465 was cloned from a cDNA library constructed from the lyophilised secretion of Hylarana guentheri. A replijavascript:void(0);cate of peptide was produced through solid phase peptide synthesis. By use of RP- HPLC and MALDI-TOF MS, the synthesised peptide was purified and its mass charge ratio was further confirmed.