Omics · study · 2026
Translational Control of Splicing Factors Directs Hematopoietic Stem Cell Fate [scRNA-seq]
Listed in NCBI GEO
The transition of hematopoietic stem cells (HSCs) from quiescence to differentiation is essential for homeostasis.
Description
While transcriptomic control of this process has been extensively studied, the diversity of mRNA isoforms during exit from quiescence remains unexplored. Here, we identify a translationally regulated splicing program governing hematopoietic fate decisions.
Splicing factors involved in branch point recognition show dynamic protein-level regulation during HSC differentiation. Among them, Sf1 is translationally controlled via a conserved, structured 5′ UTR. Disrupting translation regulation of Sf1 skews differentiation toward stem cell and erythroid programs.
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Sf1-dependent alternative splicing affects 5′ UTRs of hematopoietic and DNA damage response genes, altering their translation and impairing DNA damage resolution. These findings reveal an unrecognized link between translational control of spliceosome components, alternative splicing, and HSC fate decisions.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE295nnn/GSE295107 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE295107 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1253547 ncbi.nlm.nih.gov/bioproject/PRJNA1253547 ↗
project · from NCBI GEO
- PubMed 42345356 pubmed.ncbi.nlm.nih.gov/42345356 ↗
publication · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Mus musculus · Other
- From keywords
- Life Sciences
- Inferred from text
- Genetics 69% · Single-cell RNA sequencing 65%
Provenance · 1 source records, 10 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE295107 | 13 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].anzsrc:group:3105 | enrichment · NCBI GEO | taxonomy-embedding@1.1.0 | title+keywords+description (69%) |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[method].geo_series_type:other | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:single-cell-rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (65%) |
| concepts[organism].NCBITaxon:10090 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |