Omics · study · 2026
Jag1-regulated hybrid-EMT state identifies a subset of CD9hi pancreatic tumour-initiating cells with enriched stemness
Listed in NCBI GEO
We previously identified a pancreatic ductal adenocarcinoma (PDAC) subpopulation capable of initiating PDAC and giving rise to PDAC heterogeneity, marked by the tetraspanin CD91.
Description
PDAC shows great cellular heterogeneity, with pronounced epithelial and mesenchymal cancer cell populations, and it has been suggested that cancer cells with hybrid epithelial-mesenchymal transition (EMT) features peculiarly contribute to tumour aggressiveness2,3.
Here, we identify a stable hybrid-EMT subpopulation that are highly enriched for tumour initiating cell (TIC) properties which constituted a subpopulation of CD9hi TICs1. Hybrid-EMT CD9hi TICs showed increased capacity to form organoids and generate epithelial and mesenchymal tumour cell progeny. Interestingly, in contrast to the majority of PDAC organoids with cystic morphology, hybrid-EMT organoids exhibited a solid morphology and were able to give rise to both cystic and filled organoids, implying a relationship between stemness and solid morphology.
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Cell depletion of hybrid-EMT cells led to gradual collapse of organoids formation capability, suggesting hybrid-EMT cells are required for long-term organoid formation. Mechanistically, hybrid-EMT cells upregulated Jag1, which promoted their self-renewal by activating the Notch signalling pathway. Thus, the hybrid EMT state is a defining feature of stem cells in PDAC.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE245nnn/GSE245736 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE245736 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1029638 ncbi.nlm.nih.gov/bioproject/PRJNA1029638 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Mus musculus
- From keywords
- Life Sciences
- Inferred from text
- Cancer 75%
Provenance · 1 source records, 8 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE245736 | 13 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[disease].local:disease:cancer | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[organism].NCBITaxon:10090 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |