Omics · study · 2026
NOVEL HEXOKINASE-2 ASSOCIATED INFLAMMATORY PROCESSES IN THE GLIOBLASTOMA TUMOUR MICROENVIRONMENT
Listed in NCBI GEO
Deregulation of cellular metabolism is a hallmark of cancer, in which tumour cells upregulate glycolysis and preferentially ferment glucose to lactate (rather than pyruvate) in the 'Warburg effect'.
Description
Tumour-specific upregulation of hexokinase 2 (HK2), which catalyses the first rate-limiting step of glycolysis, has been established as key to this Warburg metabolism. Interrogating the impact of HK2 expression inhibition upon the glioblastoma transcriptome has the potential to uncover a number of novel canonical/non-canonical relationships that could serve as future co-therapeutic targets.
To investigate this, we employed RNAseq to compare the expression profiles of glioblastoma cell cultures transfected with either HK2-targeted or non-targeting negative-control siRNA, thereby identifying genes differentially expressed specifically following HK2KD. Gene ontology category enrichment (GOSeq, Enrichment map) and STRING protein-protein interaction analysis of genes identified to be differentially expressed post HK2 knockdown (HK2KD) highlighted potential non-canonical roles for HK2 in regulating inflammatory, immune and angiogenesis-related processes within the glioblastoma tumour microenvironment.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE325nnn/GSE325873 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE325873 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1442097 ncbi.nlm.nih.gov/bioproject/PRJNA1442097 ↗
project · from NCBI GEO
- PubMed 42382743 pubmed.ncbi.nlm.nih.gov/42382743 ↗
publication · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Homo sapiens
- From keywords
- Life Sciences
- Inferred from text
- Biochemistry and cell biology 71% · Cancer 75% · RNA sequencing 65%
Provenance · 1 source records, 10 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE325873 | 14 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[disease].local:disease:cancer | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[field].anzsrc:group:3101 | enrichment · NCBI GEO | taxonomy-embedding@1.1.0 | title+keywords+description (71%) |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (65%) |
| concepts[organism].NCBITaxon:9606 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |