<p>Newly identified glia-specific genes.</p>

<p><b>(A)</b> Pearson correlation coefficient of larval clusters from Konstantinides <i>and colleagues</i>, 2022 with elav-positive bulk transcriptome (x-axis) and repo-positive transcriptome (y-axis) from Özel <i>and colleagues</i>, 2021. <b>(A’)</b> UMAP representation of all cells in the larval dataset from Konstantinides <i>and colleagues</i>, 2022. Glial cells are in red and nonglial cells in dark gray. <b>(B)</b> Table showing the top differentially expressed genes between Glial and NonGlial cells. <b>(C)</b> Dot plot representing the expression of all genes from (B) by glial clusters in the larva dataset. <b>(D)</b> Combined HCR-RNA FISH and antibody staining for detection of both <i>repo</i> mRNA (cyan and separate channel below) and Repo protein (magenta and separate channel below).

Note that surface glia presents a strong protein staining but weak mRNA signal. <b>(E)</b> Double staining of AnxB9 (cyan and separate channel to the right) and Repo (magenta) antibodies. <b>(F)</b> Double staining of CG32032 (cyan and separate channel to the right) and Repo (magenta) antibodies. <b>(G)</b> Dot plot showing all nonannotated clusters from Kurmangaliyev <i>and colleagues</i>, 2020—a late pupa (P96) Optic Lobe dataset.