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Data · dataset · 2024

Experimental evidence of D-glutamate racemase activity in the uncultivated CPR bacterium Saccharimonas aalborgensis

Listed in Teesside University Research Data Repository

ORIGINAL AND RAW DATA associated with the article by Peñalver et al. "Experimental evidence of D-glutamate racemase activity in the uncultivated CPR bacterium Saccharimonas aalborgensis".

Description

In this study a glutamate (Glu) racemate from an uncultivated bacteria was expressed in a Salmonella D-Glu auxotroph and proved to complement the phenotype. Peptidoglycan structure of the Salmonella strain expressing this racemase was also characterised.

RAW DATA OF FIGURE 1: shown in SUPPLEMENTARY MATERIAL Tables S1-S2-S3. FIGURE 2: accession numbers of protein structures indicated in figure legend RAW DATA FIGURE 3: Original Western images and Petri dishes photographies. RAW DATA FIGURE 4: HPLC of in vitro assays with purified racemases.

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Files from CHROMELEON software. Includes data shown in Figure S4 Supplementary Material. RAW DATA FIGURE 5: HPLC of peptidoglycan samples (2 biological replicates) from the indicated Salmonella strains.

Files from CHROMELEON software. RAW DATA FIGURES 6-7-8-9: proteomic data deposited in the MassIVE repository (massive.ucsd.edu/ProteoSAFe/static/massive.jsp) under the accession MSV000093018. RAW DATA OF SUPPLEMENTARY MATERIAL: Includes data shown in Figures S1, S2 and S3.

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Teesside University Research Data Repositoryoai:data.mendeley.com/t8f52c7ysx.110 d agoJSON v1
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