Data · dataset · 2026
Assessing bioactivity of a bioactive peptide from the defensive skin secretion of Odorrana schmackeri
Listed in ZivaHub and Deakin Research Online and DMU Figshare — shown once because both records carry DOI 10.17034/32862557.v1
Amphibian skin is a rich source of biologically active compounds that are assumed to have diverse physiological and defence functions.
Description
In addition to the range of pharmacologically active peptides present, skin secretions contain a broad spectrum of antimicrobial peptides (AMPs). So far, these peptides have been studied in only a few species and study of additional species is expected to find further new AMPs.
Natural AMPs isolated from the amphibian skin secretions could lead studies of chemical structure analysis and synthesis of novel antimicrobials.<br><br>In this thesis, the lyophilised skin secretion of Odorrana schmackeri was obtained to examine the structure and bioactivity of the diverse peptides. The biosynthetic precursor cDNA sequence of a peptide, namely Brevinin-1-SHa, was identified through the method of “shotgun” cloning.
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The mature peptide structure was deduced from this cloned cDNA and isolated from the skin secretion sample by reverse-phase HPLC fractionation. The molecular weight of the peptide was then confirmed by using MALDI-TOF mass spectrometry. Solid phase peptide synthesis (SPPS) was employed to chemically synthesise the peptide.
Finally, antimicrobial, anticancer and haemolytic assays were carried out to assess the bioactivity of the peptide.<br><br>Structural characterisation of Brevinin-1-SHa showed that it belonged to the known family of Brevinin-1 peptides, which is widely distributed in the genus Rana. The bioactivity assays indicated that Brevinin-1-SHa is a very valuable antimicrobial peptide which has both antibacterial and anticancer activity and is deemed to be non-haemolytic at its effective concentrations.
Links
Where it is published
- DOI doi.org/10.17034/32862557.v1 ↗
DOI / persistent id · from zivahub uct ac za
Catalogue records · 1
- OAI-PMH record api.figshare.com/v2/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=oai%3Af… ↗
metadata API · from zivahub uct ac za
Topics
- From keywords
- Earth & Environmental Science · Earth & Environmental Science · Earth & Environmental Science
- Inferred from text
- Mass spectrometry 75%
Provenance · 3 source records, 8 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| ZivaHub | oai:figshare.com:article/32862557 | 10 d ago | JSON v1 |
| Deakin Research Online | oai:figshare.com:article/32862557 | 10 d ago | JSON v1 |
| DMU Figshare | oai:figshare.com:article/32862557 | 10 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| concepts[field].local:field:earth-environmental | mapping · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · figshare dmu ac uk | connector:figshare_dmu_ac_uk@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · dro deakin edu au | connector:dro_deakin_edu_au@1.0.0 | |
| concepts[modality].local:modality:mass-spectrometry | enrichment · zivahub uct ac za | keyword-concept-rules@1.0.0 | title+description (75%) |
| description | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | /metadata/dc/description |
| license_text | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| publication_date | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| title | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | /metadata/dc/title |