Data Sheet 1_Spatial learning strengthens hippocampal but not prefrontal influence on nucleus reuniens in rats.pdf

The midline thalamic nucleus reuniens (RE) is reciprocally connected with both regions and has been proposed to support HC-PFC communication.</p>Methods<p>Here, we examined local and long-range neural dynamics within the HC-RE-PFC circuit while adult male rats learned to navigate a complex maze across multiple days.

Using spectral power, coherence, and Granger causality analyses within linear mixed-effects models to control for locomotion.</p>Results<p>We observed learning-related decreases in theta power across all regions, despite increased velocity with better task performance. In contrast, beta (15–30 Hz) increased in HC but decreased in RE and PFC. Notably, HC-to-RE information flow in theta, beta, and low-gamma bands increased with learning, while RE-to-HC influences decreased.