Remodeled mechanical niches driving CXCR5 expression in paracortical CD8+ T cells dictates immunosurveillance of lymph node metastasis

ATAC-seq libraries were prepared following standard protocols from single-cell suspensions, with intact nuclei obtained through cell-membrane lysis and organellar DNA effectively removed. The study revealed significantly enhanced chromatin accessibility at the Ikzf1 locus in CD8+CXCR5+ T cells.

Ikzf1 encodes the transcription factor Ikaros, which plays critical regulatory roles in lymphocyte development and function. This dataset unveils the subset-specific epigenetic landscape of CXCR5+CD8+ T cells within the tumor microenvironment, providing valuable resources for understanding the molecular mechanisms underlying CD8+ T cell heterogeneity and functional differentiation in anti-tumor immune responses.