<p>Cluster identity in the integrated dataset and the origin of lobula neuropil glia.</p>

<p><b>(A, B)</b> UMAP representing the integration of all glial cells from L3, P15, P30, P50, P70, and adult stages (see <a href="plosbiology.org/article/info:doi/10.1371/journal.pbio.3004015#pbio.3004015.g004" target="_blank">Fig 4A</a>). Only larval cells are colored in (A), and adult cells in (B). Note that pseudocartridge, fenestrated, and chalice glia are not present in the L3 dataset (dashed circles) and the expansion of neuropil glia cell types. <b>(C)</b> <i>Hand-T2A</i>-Gal4 drives the expression of a <i>memory-lacZ</i> cassette. β-Gal (cyan and separate channel below) positive cells all express Repo (magenta), and are located in the proximal, lateral, and distal surfaces of the medulla neuropil, labeled by anti-Brp antibody (yellow and yellow dashed line below). <b>(D)</b> Section of a third instar larva optic lobe showing the lobula complex neuropil (Ncad, yellow and dashed contour).

The border between the central brain (CB) and the optic lobe (OL) is shown by a dashed line. The expression of UAS-LifeActGFP (cyan, and separate channel to the right) under the control of the <i>54H11</i>-Gal4 driver labels numerous cells that cross from the CB towards the OL and associate with the lobula complex neuropil. Repo antibody (magenta, and separate channel to the right) is used to label glial cells. <b>(E, F)</b> UMAP showing the integration of the clusters we identified as lobula neuropil glia.