Parallelised detection of bacterial viability using an electrode array and the Exeter Multiscope - dataset

<p dir="ltr">This is the supplemental data for <b>Parallelised detection of bacteria viability using an electrode array and the Exeter Multiscope</b>, published in Scientific Reports, volume 16, article 29903. doi.org/10.1038/s41598-026-66381-9</p><p dir="ltr">The research is summarised below:</p><p dir="ltr">Antimicrobial resistance remains a global existential threat. Given that antimicrobial therapy commonly starts before pathogen identification, rapid and scalable methods capable of determining effective antimicrobial compounds are needed.

In this paper, we demonstrate a 2 × 2 array of parallelised microscopes that uses low numerical aperture (NA=0.25) detection optics and LED excitation to determine bacterial viability based on their fluorescence response to an electrical stimulus. Following a 2-hour incubation, the fluorescent viability readout requires less than one minute. We use K-means clustering to classify pixels in a time lapse sequence of widefield fluorescence images and extract changes seen within bacterial clusters.