Omics · study · 2026
Transcriptomic analysis of U-373MG exposed to synthetic ShK-like peptide from the Jellyfish
Listed in NCBI GEO
Jellyfish-derived ShK-like peptides are potent blockers of the voltage-gated potassium channel Kv1.3, which plays key roles in immune cell activation and glioblastoma cell physiology.
Description
In this study, we investigated the transcriptomic responses of U-373MG human glioblastoma cells exposed to two synthetic ShK-like peptides, NnK-1 (derived from Nemopilema nomurai) and CBRV1-10493 (derived from Carybdea brevipedalia), at 200 nM for 48 h (n = 3 per group).
Since neither peptide affected cell viability at this concentration, 200 nM was selected as the treatment dose. Gene expression profiling was performed using QuantSeq 3' mRNA-Seq on an Illumina NextSeq 550 platform. Differentially expressed genes (DEGs) were identified using EdgeR with TMM+CPM normalization (fold change ≥ 1.5, p-value < 0.05).
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Exposure to NnK-1 resulted in 149 DEGs, with GO and IPA analyses indicating significant alterations in cell signaling and immune-related functions, including predicted inhibition of neuroinflammatory and immune response pathways associated with neurodegenerative diseases. Exposure to CBRV1-10493 identified 107 DEGs, with GO analysis revealing categories related to neurotransmission. These findings provide insights into the neuro-modulatory and immunological mechanisms of jellyfish ShK-like peptides in glioblastoma cells, and suggest their potential as candidates for therapeutic research in neurodegenerative and immune diseases.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE333nnn/GSE333392 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE333392 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1471025 ncbi.nlm.nih.gov/bioproject/PRJNA1471025 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Homo sapiens
- From keywords
- Life Sciences
Provenance · 1 source records, 7 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE333392 | 15 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[organism].NCBITaxon:9606 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |