Supplementary tables for "<b>Pheromone circuits and transcriptional cascades modulating transcriptional and chromatin states in the </b><b><i>Drosophila </i></b><b>central brain with social experience</b>"

Our findings suggest that social experience affects multiple genes in the central brain. Disrupting Or47b, Or67d, Fru^M, and Dsx^M function moderated the transcriptional responses through antagonistic interactions. Specifically, Or47b circuits showed a primary association with transcriptional responses to social isolation in conjunction with Dsx^M function, while Or67d and Fru^M were linked to the regulation of responses to group housing.

Notably, mutants of <i>fru</i>^<em>M</em> and <i>dsx</i>^<em>M</em> exhibited more extensive transcriptional changes in the brain than Or mutants, especially for Fru^M/Dsx^M target genes. While social experience did not lead to detectable alterations in the overall chromatin profile in the whole brain, mutants of the four genes resulted in significant changes in the enrichment of H3K4me3 and RNA polymerase II (RNAPolII) compared to wild type.

Furthermore, mutants in <i>fru</i>^<em>M</em> and <i>dsx</i>^<em>M</em> generally eliminated social experience-dependent changes in sleep and locomotion behaviors, whereas Or mutants exhibited more modest disruptions. Overall, our results uncover the pheromone circuits and transcriptional cascades in regulating molecular and behavioral responses to social experience.</p>