Data from: Effects of emodepside on single-channel properties of Onchocerca volvulus SLO-1A (BK) potassium channels

Emodepside’s effects were tested at 0.3 µM and 1.0 µM. Ovo-SLO-1A had a main open-state conductance of 110 ± 3 pS and frequent flickering sub-conductance states. The presence of the flickering sub-conductance states suggests that there is limited cooperativity between the tetrameric channel subunits required for opening to the main open-state. Emodepside increased mean current amplitudes.

Emodepside also increased open-burst times, and open probability. Verruculogen (1 µM) inhibited channel opening in the presence or absence of emodepside. This study successfully expressed Ovo-SLO-1A in HEK 293 cells, measured the conductance of the main open-state and detected the presence of sub-conductance states, and flickering openings.

The increased amplitudes of the single-channel currents, open-burst times and open probabilities provide insights into how emodepside increases Slo-1K currents and illustrate dynamic actions of emodepside on Ovo-SLO-1A.