Data · dataset · 2026
Molecular cloning, synthesis and bioactivity evaluation of a peptide from the skin secretion of the Australian tree frog, Litoria cearulea
Listed in ZivaHub and Deakin Research Online and DMU Figshare — shown once because both records carry DOI 10.17034/32638200.v1
Natural products, derived from plants, animals and minerals, have long been used in traditional medicines for the treatment of human diseases and play an important role in the modern pharmaceutical industry.
Description
Among these natural products, amphibian skin secretions serve as valuable sources for researching new medicines due to their rich variety of bioactive compounds. In this study, a bioactive peptide, namely QUB-2309, was characterised from the skin secretion of the Australian tree frog, Litoria caerulea, and its precursor cDNA sequence was obtained by 'shotgun' cloning.
Subsequently, the peptide was synthesised by solid-phase peptide synthesis. The synthetic peptide was purified by reverse-phase high-performance liquid chromatography and identified by MOLDI-TOF mass spectrometry. To assess the antibacterial activity, anticancer activity and haemolytic activity of the peptide, MIC/MBC assays, MTT assay and haemolysis assay were performed.
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QUB-2309 demonstrated statistically significant inhibitory activity against human lung cancer cell line NCI-H838 staring from a concentration of 10-5 M. However, no antimicrobial activity was detected against the tested strains, including Escherichia coli, Staphylococcus aureus and Candida albicans, at the highest tested concentration. Additionally, QUB-2309 has no detectable haemolytic activity even at a concentration of 512 μM. The lack of hemolytic activity suggests that QUB-2309 may have a favourable safety profile, making it a promising candidate for further development as a therapeutic agent.<br><br><i>Thesis is embargoed until 31 December 2028.</i>
Links
Where it is published
- DOI doi.org/10.17034/32638200.v1 ↗
DOI / persistent id · from zivahub uct ac za
Catalogue records · 1
- OAI-PMH record api.figshare.com/v2/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=oai%3Af… ↗
metadata API · from zivahub uct ac za
Topics
- From keywords
- Earth & Environmental Science · Earth & Environmental Science · Earth & Environmental Science · Life Sciences · Life Sciences · Life Sciences
- Inferred from text
- Agricultural biotechnology 71% · Cancer 75% · Mass spectrometry 75%
Provenance · 3 source records, 14 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| ZivaHub | oai:figshare.com:article/32638200 | 11 d ago | JSON v1 |
| Deakin Research Online | oai:figshare.com:article/32638200 | 11 d ago | JSON v1 |
| DMU Figshare | oai:figshare.com:article/32638200 | 11 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| concepts[disease].local:disease:cancer | enrichment · zivahub uct ac za | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[field].anzsrc:group:3001 | enrichment · zivahub uct ac za | taxonomy-embedding@1.1.0 | title+keywords+description (71%) |
| concepts[field].local:field:earth-environmental | mapping · dro deakin edu au | connector:dro_deakin_edu_au@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · figshare dmu ac uk | connector:figshare_dmu_ac_uk@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · dro deakin edu au | connector:dro_deakin_edu_au@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · figshare dmu ac uk | connector:figshare_dmu_ac_uk@1.0.0 | |
| concepts[modality].local:modality:mass-spectrometry | enrichment · zivahub uct ac za | keyword-concept-rules@1.0.0 | title+description (75%) |
| description | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | /metadata/dc/description |
| license_text | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| publication_date | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| title | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | /metadata/dc/title |