Comparative study in gene expression of type 2 cytokine secretion activity of Group 2 innate lymphoid cells (ILC2s) isolated from nasal polyps or peripheral blood of patients with chronic eosinophilic rhinosinusitis with nasal polyps

To investigate the differences in type 2 cytokine secretion activity of ILC2s by original location in vivo, we performed time series measurements of type 2 cytokine secretion activity of ILC2 after IL-33 stimulation on ILC2 isolated from peripheral blood or nasal polyps sampled from patients with with chronic eosinophilic rhinosinusitis. Secretion-negative or -positive cells after stimulation were collected separately and performed RNA-seq on each cell for gene expression profiling analysis.