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Data · dataset · 2025

Microplate approach to resin screening and parameter estimation applied to chromatography column modeling of gradient elution operation

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# Microplate data used for materials screening and estimation of equilibrium parameters The experimental data whit 4 microplates containing 96 wells, white already discounted.

Description

Each plate had a different amount of resin into the wells. The buffer solutions with different concentrations of ethanol: ϕ1, ϕ2, ϕ3 in different columns.

Different amounts of resins were added into the wells of the other plates, named P1, P2, P3 and P4, each with: 10, 15, 25 and 50 mg into the plates named P1, P2, P3 and P4, respectively. Each of the 8 total rows per plate corresponded to a different type of resin. Spreadsheet P1- P4: supernatant concentration data Spreadsheet Config: experiment setup # Column chromatography estimation The experimental concentration chromatographic peak of NP40 was used to estimate the parameters.

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These data already take into account the corrections for the dead volume of 5.8 mL and the permanence volume of 10.94 mL.

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