Data · dataset · 2026
Protective effect of HUCMSCs-Exo on IFN-γ-mediated melanocyte injury
Listed in ScienceDB
Objective To explore the protective effect of exosomes derived from human umbilical cord mesenchymal stem cells (HUCMSCs-Exo) against interferon-gamma (IFN-γ)-mediated melanocyte injury.
Description
Methods
Human epidermal melanocytes were cultured in vitro. Melanocytes were treated with different concentrations of IFN-γ (100, 200, 400, 800 ng/mL) for 48 hours, and cell survival rate was detected by CCK-8 assay to screen an appropriate IFN-γconcentration for establishing an immunosuppressive model. Melanocytes were treated with different concentrations of HUCMSCs-Exo for 48 hours, and cell proliferation activity was detected by CCK-8 assay to determine the safe concentration of exosomes for subsequent experiments.
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Melanocytes were pretreated with exosomes at a safe concentration and then incubated with or without 100 ng/mL IFN-γ for 48 hours. Cell proliferation activity was assessed using the CCK-8 assay; mitochondrial membrane potential was detected by JC-1 staining; apoptosis was evaluated by flow cytometry; and tyrosinase activity was measured by the dopamine oxidation method.Results With the increase in IFN-γ concentration, cell survival rate gradually decreased.
The cell survival rates in the 100, 200, 400, and 800 ng/mL IFN-γ groups were significantly different from those in the control group (P ＜ 0.05). 100 ng/mL IFN-γ was selected to establish an epidermal melanocyte immunosuppressive model. Melanocytes treated with 10 and 20 μg/mL exosomes for 48 hours showed significant differences compared with the control group (P ＜ 0.05), and 10 μg/mL exosomes were selected for subsequent experiments.
Compared with the IFN-γ group, exosomes significantly increased cell proliferation activity.Conclusions HUCMSCs-Exo has a protective effect against IFN-γ-mediated immunosuppressive injury in melanocytes.
Links
Where it is published
- DOI doi.org/10.57760/sciencedb.j00217.12028 ↗
DOI / persistent id · from scidb cn
Catalogue records · 1
- OAI-PMH record scidb.cn/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=10.57760%2… ↗
metadata API · from scidb cn
Topics
- From keywords
- Earth & Environmental Science · Engineering · Humanities · Life Sciences · Social Science
Provenance · 1 source records, 10 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| ScienceDB | 10.57760/sciencedb.j00217.12028 | 12 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:engineering | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:humanities | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:social-science | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| description | source · scidb cn | connector:scidb_cn@1.0.0 | /metadata/dc/description |
| license | source · scidb cn | connector:scidb_cn@1.0.0 | /metadata/dc/rights |
| publication_date | source · scidb cn | connector:scidb_cn@1.0.0 | |
| title | source · scidb cn | connector:scidb_cn@1.0.0 | /metadata/dc/title |