Omics · study · 2026
Single-nuclei RNA-Seq to characterize the immediate whole-animal postprandial transcriptional response following a high-fat meal in larval zebrafish
Listed in NCBI GEO
In this study, we employed a fluorescent BODIPY-fatty acid feeding assay and 10x Genomics single nuclei RNA-sequencing (snRNA-seq) to explore the simultaneous transcriptional responses following consumption of a high-fat meal in the context of a whole vertebrate organism (larval zebrafish).
Description
We used the whole-animal nuclear transcriptome sampling over short sampling periods (0 to 4 hours) to demonstrate how known metabolic pathways intertwine in new functional/spatial and immediate temporal contexts, and demonstrated the power of larval zebrafish snRNA-seq and fluorescent high-fat feeding protocols to describe the whole-animal response to consumption of a high-fat meal.
To our knowledge, such an approach has never been accomplished before in a vertebrate model.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE346nnn/GSE346015 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE346015 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1522754 ncbi.nlm.nih.gov/bioproject/PRJNA1522754 ↗
project · from NCBI GEO
Topics
- Stated by source
- Danio rerio · Expression profiling by high throughput sequencing
- From keywords
- Life Sciences
- Inferred from text
- Bioinformatics and computational biology 74% · RNA sequencing 65% · Single-cell RNA sequencing 65%
Provenance · 1 source records, 10 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE346015 | 14 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].anzsrc:group:3102 | enrichment · NCBI GEO | taxonomy-embedding@1.1.0 | title+keywords+description (74%) |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (65%) |
| concepts[modality].local:modality:single-cell-rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (65%) |
| concepts[organism].NCBITaxon:7955 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |