Data · dataset · 2025
RNA-Seq of Glischrochilus (Librodor) japonius: adult female whole body without gut and elytron
Listed in ScienceDB
Total RNA was extracted from the whole body of a single adult female G. japonius using the Trizol reagent protocol, Messenger RNA (mRNA) was then purified from the total RNA using poly-T oligo-attached magnetic beads.
Description
After purification, the RNA was fragmented, and first-strand cDNA was synthesized using random hexamer primers, followed by second-strand cDNA synthesis. The library was finalized through a series of steps, including end repair, A-tailing, adapter ligation, size selection, amplification, and purification.
The library was sequenced on the Illumina NovaSeq X Plus platform (PE 150bp), and conducted 6.7 Gb of RNA-seq clean data.
Links
Where it is published
- DOI doi.org/10.57760/sciencedb.17157 ↗
DOI / persistent id · from scidb cn
Catalogue records · 1
- OAI-PMH record scidb.cn/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=10.57760%2… ↗
metadata API · from scidb cn
Topics
- From keywords
- Earth & Environmental Science · Engineering · Humanities · Life Sciences · Social Science
- Inferred from text
- RNA sequencing 65%
Provenance · 1 source records, 11 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| ScienceDB | 10.57760/sciencedb.17157 | 9 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:engineering | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:humanities | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:social-science | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[modality].local:modality:rna-seq | enrichment · scidb cn | keyword-concept-rules@1.0.0 | title+description (65%) |
| description | source · scidb cn | connector:scidb_cn@1.0.0 | /metadata/dc/description |
| license | source · scidb cn | connector:scidb_cn@1.0.0 | /metadata/dc/rights |
| publication_date | source · scidb cn | connector:scidb_cn@1.0.0 | |
| title | source · scidb cn | connector:scidb_cn@1.0.0 | /metadata/dc/title |