Omics · study · 2026
To screen the epigentic facters' effect on mESC state transition, Naïve state, Primed state.
Listed in NCBI GEO
The transcriptional and epigenetic status restricts the self-renewal ability and lineage specificity of the naïve and primed mouse embryonic stem cells (mESC).
Description
CRISPR/Cas9-based functional screening coupled with single cell RNA-seq (CROP-seq) establishes relationships between gRNA-mediated knockout genotypes and transcriptome phenotypes, and is a novel tool to unravel gene regulatory networks. Here, we used CROP-seq approach to dissect the transcriptional and epigenetic regulation of the pluripotency network in mESCs.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE316nnn/GSE316051 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE316051 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1400531 ncbi.nlm.nih.gov/bioproject/PRJNA1400531 ↗
project · from NCBI GEO
- PubMed 42462715 pubmed.ncbi.nlm.nih.gov/42462715 ↗
publication · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Mus musculus
- From keywords
- Life Sciences
- Inferred from text
- Genetics 70% · RNA sequencing 65%
Provenance · 1 source records, 9 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE316051 | 13 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].anzsrc:group:3105 | enrichment · NCBI GEO | taxonomy-embedding@1.1.0 | title+keywords+description (70%) |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (65%) |
| concepts[organism].NCBITaxon:10090 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |