Omics · study · 2026
Lipid-based mRNA chimeric antigen receptor T cells for enhanced in vitro antitumor activity
Listed in NCBI GEO
Chimeric antigen receptor (CAR) T cell therapy provides substantial clinical benefits in hematologic malignancies.
Description
However, cell engineering using conventional viral vectors is costly, labor-intensive, and prone to safety risks, while electroporation-induced cellular damage limits manufacturing scalability. This study reports a safe, high-efficiency, nonviral platform for generating mRNA-engineered CAR-T cells.
Herein, eight ionizable lipids are synthesized and formulated into lipid nanoparticles. Systematic screening and structural optimization identified Lipid 6 as the lead candidate. Transfection efficiency is validated by delivering eGFP mRNA into DC2.4, peripheral blood mononuclear, dendritic, and natural killer cells.
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Murine models confirm robust in vivo expression and a favorable safety profile. Lipid 6–mediated delivery of MUC1 mRNA generates MUC1–CAR-T cells characterized by high surface receptor expression and potent cytotoxicity against MUC1-positive tumor cells. These findings establish Lipid 6 as a feasible strategy for primary human T-cell engineering, facilitating functional CAR expression with minimal toxicity.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE338nnn/GSE338028 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE338028 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1492132 ncbi.nlm.nih.gov/bioproject/PRJNA1492132 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Homo sapiens
- From keywords
- Life Sciences
- Inferred from text
- Cancer 65%
Provenance · 1 source records, 8 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE338028 | 13 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[disease].local:disease:cancer | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (65%) |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[organism].NCBITaxon:9606 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |