Omics · study · 2026
USP1 acts as a chaperone for HSFA2 and plays a crucial role in thermopriming in Arabidopsis
Listed in NCBI GEO
Plants employ diverse strategies to cope with different types of heat stress.
Description
The response to short-term acute heat stress differs significantly from that to prolonged, moderate heat stress followed by severe stress events. After experiencing prolonged moderate heat stress, plants exhibit a more robust response to subsequent severe stress, a phenomenon known as thermopriming or acquired thermotolerance.
Thermopriming creates a memory by maintaining the heat stress (HS) memory-related genes in an alert state. In this work, we investigated the role of Arabidopsis Universal Stress Protein 1 (USP1) in plant heat stress responses. CRISPR-Cas9 generated knockout usp1 mutant lines showed no morphological changes in development in normal growth conditions.
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However, usp1 mutant plants showed enhanced levels of apoplast hydrogen peroxide and superoxide reactive oxygen species accumulation upon heat stress. Transcriptome analyses revealed that genes related to protein folding, electron transport, and oxidative phosphorylation are strongly upregulated in usp1 mutant plants. USP1 is essential for acquired thermotolerance, as usp1 mutant plants show decreased transcript levels of heat stress response genes and reduced H3K4me3 enrichment at memory gene loci.
USP1 is involved in thermopriming by interacting with HEAT SHOCK TRANSCRIPTION FACTOR A2 (HSFA2). Biochemical assays showed that USP1 functions as a molecular chaperone, protecting the transcription factor HSFA2 from heat-induced denaturation. Moreover, usp1 mutants are compromised in heat stress memory but show normal responses to acute heat stress similar to hsfa2 mutants.
These data show that USP1 plays an important role as a chaperone of HSFA2 in mediating plant heat stress memory.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE286nnn/GSE286899 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE286899 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1210515 ncbi.nlm.nih.gov/bioproject/PRJNA1210515 ↗
project · from NCBI GEO
Topics
- Stated by source
- Arabidopsis thaliana · Expression profiling by high throughput sequencing
- From keywords
- Life Sciences
Provenance · 1 source records, 7 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE286899 | 15 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[organism].NCBITaxon:3702 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |