Data · dataset · 2026
QUB-2437: evaluation of a novel antimicrobial peptide isolated from the defensive skin secretion of the Jaguar leaf frog, Phyllomedusa palliata
Listed in ZivaHub and Deakin Research Online and DMU Figshare — shown once because both records carry DOI 10.17034/32867051.v1
Amphibians can easily live in an abundant microbial environment on account of their specific organic secretions providing a unique source of bioactive peptides.
Description
Antimicrobial peptides (AMPs) are considered as feasible alternatives to conventional antibiotics due to their low resistance and broad spectra against bacteria, fungi and even cancers. <br>In present study, a novel natural dermaseptin peptide, preliminarily named as QUB-2437, was identified from the defensive skin secretion of the Jaguar Leaf Frog, Phyllomedusa palliata.
The cDNA library was constructed from skin secretion-derived mRNA. The encoded amino acid sequence of mature peptide was deduced as GLWSTIKNVGKEAAIAAGKAVLGAL-NH2 by molecular cloning technique. Subsequently, QUB-2437 was synthesised using SPPS, and RP-HPLC was employed to purify the synthetic product.
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The molecular weight of QUB-2437 was confirmed by MALDI-TOF MS. Afterwards, antimicrobial assays, MTT cell proliferative assay as well as haemolytic assay were carried out to evaluate its bioactivity and cytotoxicity. The novel QUB-2437 exhibited different antimicrobial activities against three strains of microorganism including S. aureus, E. coli and C. albicans with MIC values of 16, 8 and 128 μM, and MBC values of 16, 8 and 256 μM, respectively.
It was also discovered that there was an inhibition against four human cancer cell lines NCI-H157, U-251 MG, PC-3 and MCF-7. However, a relatively strong cytotoxicity on horse erythrocytes was observed as well. QUB-2437 possessed up to 5% haemolytic potency at concentration of 8 μM.<br>The above results indicated that structural modification would be required to reduce the cytotoxicity of the natural dermaseptin peptide for future clinical application. <br>
Links
Where it is published
- DOI doi.org/10.17034/32867051.v1 ↗
DOI / persistent id · from zivahub uct ac za
Catalogue records · 1
- OAI-PMH record api.figshare.com/v2/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=oai%3Af… ↗
metadata API · from zivahub uct ac za
Topics
- From keywords
- Earth & Environmental Science · Earth & Environmental Science · Earth & Environmental Science
- Inferred from text
- Cancer 75%
Provenance · 3 source records, 8 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| ZivaHub | oai:figshare.com:article/32867051 | 13 d ago | JSON v1 |
| Deakin Research Online | oai:figshare.com:article/32867051 | 13 d ago | JSON v1 |
| DMU Figshare | oai:figshare.com:article/32867051 | 13 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| concepts[disease].local:disease:cancer | enrichment · zivahub uct ac za | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[field].local:field:earth-environmental | mapping · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · dro deakin edu au | connector:dro_deakin_edu_au@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · figshare dmu ac uk | connector:figshare_dmu_ac_uk@1.0.0 | |
| description | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | /metadata/dc/description |
| license_text | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| publication_date | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| title | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | /metadata/dc/title |