H3K27ac CUT&Tag profiling of kidney allograft - infiltrating and matched PBMC CD8+ T cells

However, the epigenetic mechanisms underlying these tissue-specific effector states in human transplantation remain incompletely understood. Here, we performed H3K27ac CUT&Tag profiling on CD8+ T cells isolated from rejected human kidney allografts and matched peripheral blood mononuclear cells (PBMCs).

Comparative analyses revealed distinct H3K27ac enrichment patterns at loci associated with cytotoxic effector programs in allograft-infiltrating CD8+ T cells relative to matched circulating CD8+ T cells. These data provide a resource for dissecting epigenetic programs that shape pathogenic CD8+ T cell responses during human kidney allograft rejection.