Table 1_Exploratory ferroptosis-related transcriptomic patterns in dilated cardiomyopathy and pan-cancer datasets with H9c2 RT-qPCR assessment.docx

GPX4, SLC7A11, ACSL4, and TFRC mRNA were assessed by RT-qPCR in Ang II-stimulated H9c2 cells.</p>Results<p>Fourteen cluster-derived cell categories were retained. Cardiomyocytes, macrophages, and endothelial cells were not detected in the selected DCM donor, so no condition-specific inference was made for those categories. Among 109 detected ferroptosis-related genes, 60 met the cell-level up threshold, 24 met the down threshold, and 25 were nonsignificant; these counts are not donor-level evidence.

In GSE5406, 5 of 99 tested ferroptosis-related genes met |log2FC| > 0.5 and BH-adjusted p < 0.05. Fold changes for 96 detected genes were uncorrelated across platforms (r = 0.034, p = 0.740). RT-qPCR showed lower GPX4 and SLC7A11 and higher ACSL4 and TFRC mRNA, with gene- and context-specific directionality (Supplementary Table S1).</p>Conclusion<p>The analyses identify exploratory, donor- and context-dependent transcriptional patterns.

They do not establish ferroptosis as a causal mechanism, biomarker, or treatment target in primary HHD. Multi-donor disease-specific cohorts and protein-level and functional experiments are required.</p>