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Data · dataset · 2014

Beads convolution/deconvolution simulation

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Simulation of beads acquired with a 100x / 1.4 NA objective, the pixel size is 65 nm and the Z spacing is 200 nm.

Description

This is a simple "pixelated" simulation. Beads were 3 to 4 voxels laterally and 1 voxel axially.

They were convolved with a PSF generated using the above parameters and assuming an embedding media of RI=1.4 and a depth below coverslip of 1 micron. Simulated phantom was convolved with PSF to produce a simluated "image", then deconvolved with 200 iterations of noncirculant Richardson Lucy. Low level code was written in Java (deconvolution, psf generation).

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High level code was written in Jython and can be found here - github.com/bnorthan/projects/tree/master/Scripts/Jython.

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Inferred from text
Image 75% · Simulation 75%
Provenance · 1 source records, 11 field assertions
SourceKeyLast seenRaw
DataCite10.6084/m9.figshare.100216713 d agoJSON v1
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concepts[method].local:method:simulationenrichment · DataCitekeyword-concept-rules@1.0.0title+description (75%)
concepts[modality].local:modality:imageenrichment · DataCitekeyword-concept-rules@1.0.0title+description (75%)
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