Omics · study · 2026
scRNA-seq of immune and structural cells in hypercholesterolemic mice
Listed in NCBI GEO
We performed scRNA-seq analysis using the 10X genomics platform of ApoE-/- mice fed for 16 weeks with high-fat diet (HFD).
Description
In these mice, we isolated brachiocephalic artery (BCA, digested), blood, bone marrow, spleen as well as perfusates of lung and liver at two times of the day (ZT1 and ZT13). For BCA cells, not-enriched cells and enriched cells (neutrophils, B- and T-cells, F4/80+ macrophages, CD31+ endothelial cells and CD31- stromal cells) were isolated and loaded for processing.
For blood, bone marrow, spleen and the lung and liver perfusates, Lineage- Gr1+ were sorted together with enriched cKit+ cells.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE316nnn/GSE316605 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE316605 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1403458 ncbi.nlm.nih.gov/bioproject/PRJNA1403458 ↗
project · from NCBI GEO
- PubMed 41996475 pubmed.ncbi.nlm.nih.gov/41996475 ↗
publication · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Mus musculus · Other
- From keywords
- Life Sciences
- Inferred from text
- Single-cell RNA sequencing 65%
Provenance · 1 source records, 9 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE316605 | 15 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[method].geo_series_type:other | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:single-cell-rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (65%) |
| concepts[organism].NCBITaxon:10090 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |