<p>SARS-CoV-2 variants entry and binding with a library of bat ACE2.</p>

Key regions of the N-terminal domain (NTD), receptor-binding domain (RBD), S1 and S2 are highlighted and the mutations that arose in these variants have been annotated. The BA.4 and BA.5 Spike is identical, and the difference in XBB1.5 compared to XBB is denoted by an asterisk, which is a reversion of the mutation at position 494 (R493Q). <b>(B)</b> Infection of HEK293T cells stably expressing hACE2 with SARS-CoV-2, WT and BA.1 pseudoparticles, along with individual mutants generated of these variants at positions 417, 484, 493 and 501, either singly or in combination.

Virus was titrated 5-fold on cells and Firefly luciferase signals measured; a negative control (non-enveloped, NE) was also included. The data underlying this Figure can be found in <a href="plosbiology.org/article/info:doi/10.1371/journal.pbio.3003944#pbio.3003944.s017" target="_blank">S1 Data</a>.</p> <p>(TIF)</p>