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Data · dataset · 2026

<p>Regulation of the CSP and glycation by the stringent response and c-di-GMP signaling pathways.</p>

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Description

<p>Wild-type (WT), <i>rel</i><sub>Bbu</sub> mutant (∆<i>rel</i><sub>Bbu</sub>) (A), <i>dksA</i> mutant (∆<i>dksA</i>) (B), <i>rrp1</i> mutant (∆<i>rrp1</i>) (C), or <i>plzA</i> mutant (∆<i>plzA</i>) (D) and respective complemented strains were grown to ~7 × 10<sup>7</sup> cell ml<sup>-1</sup> in normal BSK + RS growth medium and whole cell lysates collected or shifted to nutrient stress medium containing glycerol (RPMI + glycerol) or neopeptone (RPMI + neopeptone) for 24 h before collecting cell lysates.

Samples were separated by SDS-PAGE and analyzed by immunoblot using antibodies against MG (to recognize glycated proteins), MgsA and FlaB.</p>

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ZivaHuboai:figshare.com:article/3402690013 d agoJSON v1
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