Excel · study · 2026
SETDB2 inhibits thyroid cancer progression by regulating the expression of the splicing factor U2AF1
Listed in NCBI GEO
Objective: This study aimed to investigate SETDB2 expression, clinical significance, and molecular mechanism in thyroid cancer progression.
Description
Methods
SETDB2 expression and prognosis were analyzed using TCGA and CPTAC databases, and validated by immunohistochemistry in 90 clinical samples (primary, recurrent, metastatic papillary, and anaplastic carcinoma). Overexpression and knockdown models were established in BCPAP and BHT101 cells. Proliferation was assessed by CCK‑8, colony formation, and EdU assays.
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RNA‑seq and qPCR identified downstream targets, with rescue experiments via U2AF1 overexpression. Results: SETDB2 was significantly downregulated in recurrent and metastatic papillary and anaplastic thyroid carcinoma, and low expression correlated with poor prognosis. SETDB2 overexpression suppressed proliferation and colony formation in anaplastic BHT101 cells, whereas knockdown promoted growth in papillary BCPAP cells.
U2AF1 was identified as a key downstream target, and its overexpression partially reversed the inhibitory effects of SETDB2 on proliferation. Conclusion: SETDB2 acts as a tumor suppressor in thyroid cancer; its loss promotes progression, likely through regulating U2AF1. The SETDB2‑U2AF1 axis represents a potential therapeutic target.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE342nnn/GSE342848 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE342848 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1508972 ncbi.nlm.nih.gov/bioproject/PRJNA1508972 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Homo sapiens
- From keywords
- Life Sciences
- Inferred from text
- Cancer 75%
Provenance · 1 source records, 8 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE342848 | 15 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[disease].local:disease:cancer | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[organism].NCBITaxon:9606 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |