EAGER: Metabolomics Analysis of Archival Marine Invertebrates

Technical description of the project This project seeks to develop a workflow for the analysis of metabolites in archival marine invertebrate specimens held in the Smithsonian National Museum of Natural History (NMNH). Recent advances in mass spectrometry (MS) and nuclear magnetic resonance (NMR) spectroscopy, both instrumental as well as analysis platforms, enable the detection and annotation of chemical structures in these otherwise difficult to obtain metabolites.

In particular, NMR strategy (Pure Shift NMR) will be implemented to increase sensitivity toward these sample-limited analytes. Further, the workflow will be applied in an analysis of storage methods used by the NMNH with the aim of understanding how best to preserve specimens for future metabolomics analyses. With an optimized workflow established, additional applications to inform our understanding of adaptation and (cryptic) speciation in the extreme habitats found in Antarctica are possible.

This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.