Omics · study · 2026
Effect of DOT1L inhibition on gene expression in SW1990 pancreatic cancer cells
Listed in NCBI GEO
Histone methyltransferase DOT1L plays a critical role in the regulation of gene expression through methylation of histone H3 at lysine 79 (H3K79), a modification associated with active transcription.
Description
However, its function in pancreatic ductal adenocarcinoma (PDAC) remains incompletely understood. In this study, we investigated the transcriptional impact of DOT1L in SW1990 pancreatic cancer cells using EPZ-5676, a selective DOT1L inhibitor.
Treatment with EPZ-5676 resulted in widespread transcriptional changes, indicating a key regulatory role of DOT1L in maintaining the gene expression profile of SW1990 cells. Through RNA-seq analysis, we identified differentially expressed genes between the untreated control and EPZ-5676-treated cells, implicating DOT1L in pathways involved in cell proliferation, differentiation, and tumor progression. These findings suggest that DOT1L-mediated H3K79 methylation contributes to the transcriptional landscape of pancreatic cancer cells and may represent a potential therapeutic target.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE303nnn/GSE303903 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE303903 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1298197 ncbi.nlm.nih.gov/bioproject/PRJNA1298197 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Homo sapiens
- From keywords
- Life Sciences
- Inferred from text
- Cancer 75% · Genetics 68% · RNA sequencing 65%
Provenance · 1 source records, 10 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE303903 | 15 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[disease].local:disease:cancer | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[field].anzsrc:group:3105 | enrichment · NCBI GEO | taxonomy-embedding@1.1.0 | title+keywords+description (68%) |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (65%) |
| concepts[organism].NCBITaxon:9606 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |