Data · dataset · 2022
Immunofluorescence data of 700 host cell proteins in Sars-CoV2 infected vs non-infected VeroE6 cells
Listed in Researchdata.se
The data set contains ≈ 75,000 confocal images of mixed Vero E6 cell populations (SARS-CoV2 infected vs non-infected).
Description
Cells had been infected with the virus for 1 h and fixed 24 hour post infection in 96 well plates. Using immunofluorescence (4 colours) cells were stained for the SARS-CoV2 virus (red), the endoplasmic reticulum (yellow) and nucleus (blue) – same for all images in the data set and used as reference markers.
On top of this, 700 different target proteins were stained – one per well (green). Images were acquired with an Opera Phoenix microscope at 63X, using 9 FoV and 3 z-planes per protein target. IN the original study, intensity differences and spatial re-distribution of the target proteins were evaluated in infected vs the non-infected cells.
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The image resource was generated and described in detail in this research article: (to be added).
Links
Where it is published
- DOI doi.org/10.17044/scilifelab.14315777 ↗
DOI / persistent id · from researchdata se
Catalogue records · 1
- OAI-PMH record api.researchdata.se/oai-pmh?verb=GetRecord&metadataPrefix=oai_dc&identifier=oai%3A… ↗
metadata API · from researchdata se
Topics
- From keywords
- Earth & Environmental Science · Medicine & Health
- Inferred from text
- Image 75% · Immunology 69%
Provenance · 1 source records, 9 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| Researchdata.se | oai:researchdata.se:doi-10-17044-scilifelab-14315777/0 | 10 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · researchdata se | connector:researchdata_se@1.0.0 | |
| concepts[field].anzsrc:group:3204 | enrichment · researchdata se | taxonomy-embedding@1.1.0 | title+keywords+description (69%) |
| concepts[field].local:field:earth-environmental | mapping · researchdata se | connector:researchdata_se@1.0.0 | |
| concepts[field].local:field:medicine-health | mapping · researchdata se | connector:researchdata_se@1.0.0 | |
| concepts[modality].local:modality:image | enrichment · researchdata se | keyword-concept-rules@1.0.0 | title+description (75%) |
| description | source · researchdata se | connector:researchdata_se@1.0.0 | /metadata/dc/description |
| license | source · researchdata se | connector:researchdata_se@1.0.0 | /metadata/dc/rights |
| publication_date | source · researchdata se | connector:researchdata_se@1.0.0 | |
| title | source · researchdata se | connector:researchdata_se@1.0.0 | /metadata/dc/title |