Polysaccharide from Polygonatum cyrtonema Hua ameliorates alcoholic liver injury by activating the AMPKα/SIRT1/PGC-1α signaling pathway-Original data

This study aims to investigate the ameliorative effects and underlying mechanisms of Polygonatum cyrtonema Hua polysaccharide 1 (PCP1) on alcohol-related liver disease (ALD) in mice, with a focus on analyzing the regulatory role of the adenosine 5'-monophosphate-activated protein kinase α (AMPKα)/silent information regulator 1 (SIRT1)/peroxisome proliferator-activated receptor gamma coactivator-1 alpha (PGC-1α) signaling pathway.

An ALD mouse model was established using chronic alcohol feeding and acute alcohol gavage, and the mice were randomly divided into a control group, model group, positive drug group, PCP1 low-dose group, and PCP1 high-dose group. Liver pathological changes were evaluated through HE staining, oil red O staining, and immunohistochemistry. Biochemical methods were used to measure liver function indicators such as serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), total cholesterol (TC), and triglyceride (TG) levels, while ELISA was employed to determine the levels of inflammatory cytokines tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6).