Enterovirus 71 infection induces pyroptotic brain injury via synergistic activation of classical inflammasome and viral gInfection Induces Pyroptotic Brain Injury via Synergistic Activation of Classical Inflammasome and Viral Gasdermin D cCleavage

This dataset, which supports a study investigating enterovirus 71 (EV71) -induced arson brain injury, contains multimodal experimental data for five main illustrations and supplementary material.Data generation and processing: An animal model was established by infecting BALB/c mice (6-8 weeks old) and using uninfected mice as controls. Tissues and samples were collected at multiple time points and performed in parallel in vitro experiments using a human neural cell line (RD).

For data generation:Figure 1 includes: (A) raw images of the preparation process of the animal model before sampling; (B) tabulated data of mouse body weight change and disease score assessment (with columns of "time point", "group", "body weight (g)" and "disease score"); (C) original Western blot band images and gray intensity statistics; (D) Raw images with HE and Nissl staining.Figure 2 contains the raw four-color immunofluorescence images and colocalization statistics, which analyze the spatial relationships of target proteins in terms of colocalization coefficients.Figure 3 contains: (A) raw Western blot band images and gray-scale intensity statistics; (B) original immunofluorescence images and optical density statistics of pre-Il-1β + cleaved IL-1β; (C) Raw immunofluorescence images and optical density statistics of VP-1 + IL-18.Figure 4 includes: (A) statistics for body weight and disease score; (B) Raw images with HE and Nissl staining.Figure 5 contains: (A) the original Western blot band image and gray intensity statistics; (B) original immunofluorescence images and optical density statistics of pre-caspase-1 + cleaved caspase-1; (C) Raw immunofluorescence images and optical density statistics of GSDMD + Caspase-11.The supplementary figure presents the original image of the cell state in the in vitro assay.Data processing involved: quantification of Western ink gray scale and immunofluorescence optical density using ImageJ; Statistical software (GraphPad Prism) was used to analyze body weight, disease score, and colocalization/optical density data.