Data · dataset · 2019
Microarray data for microRNA-196a KD in immortalised abdominal and gluteal pre-adipocytes.
Listed in Teesside University Research Data Repository
Microarray data for pre-adipocyte cell lines imAPAD-mir-196aKD, imAPAD-Con, imGPAD-mir196aKD and imGPAD-Con.
Description
Six replicates of passage 10 imAPAD mir-196aKD, imAPAD-Con, imGPAD mir-196aKD and imGPAD-Con pre-adipocytes were harvested in Tri-reagent. Cells were harvested on day 3 after plating and were sub-confluent.
RNA was isolated using the mirVana miRNA Isolation Kit (Life Technologies). RNA concentration was assessed using the Nanodrop ND-1000 spectrophotometer and by RiboGreen® assay and samples were normalised to give 100ng RNA in a total volume of 11 μl. RNA quality was verified using the Agilent TapeStation (Agilent, Santa Clara, US).
Read the rest (2 more)
RNA samples were reverse transcribed using the Illumina TotalPrep-96 RNA Amplification Kit and then converted into labelled cDNA using the Illumina Whole-Genome Gene Expression Direct Hybridisation Assay. The labelled ss-cDNA was then hybridised to two HumanGene2.1 ST-16 Array Plates. The array was washed, stained and scanned using the Affymetrix GeneTitan platform.
Raw data were Robust multi-array (RMA) normalised and checked for quality. Differential expression analysis was performed in R.
Links
Where it is published
- DOI doi.org/10.17632/m4xtmzmx39.1 ↗
DOI / persistent id · from researchdata tees ac uk
Catalogue records · 1
- OAI-PMH record data.mendeley.com/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=oai%3Adata… ↗
metadata API · from researchdata tees ac uk
Topics
- From keywords
- Earth & Environmental Science · Engineering · Gene expression · Humanities · Life Sciences · Social Science
Provenance · 1 source records, 11 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| Teesside University Research Data Repository | oai:data.mendeley.com/m4xtmzmx39.1 | 12 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | |
| concepts[field].anzsrc:field:310505 | mapping · researchdata tees ac uk | vocabulary-mapper@1.0.0 | keywords['Microarray'] |
| concepts[field].local:field:earth-environmental | mapping · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | |
| concepts[field].local:field:engineering | mapping · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | |
| concepts[field].local:field:humanities | mapping · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | |
| concepts[field].local:field:social-science | mapping · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | |
| description | source · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | /metadata/dc/description |
| license | source · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | /metadata/dc/rights |
| publication_date | source · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | |
| title | source · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | /metadata/dc/title |