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Imaging · dataset · 2018

Functions of FACT in Breaking Nucleosome and Maintaining Its Integrity at Single-nucleosome Level

Listed in Teesside University Research Data Repository

Fig 3i Gel-shift assay shows the properties of FACT, SSRP1 or SPT16 to promote the deposition of H2A/H2B dimer into tetrasome to form nucleosome.

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Fig 4a Gel-shift assay shows the DNA binding properties of SSRP1 and SSRP1ΔHMG. Fig 4b Gel-shift assay shows the properties of SSRP1 and SSRP1ΔHMG to promote H2A/H2B dimer deposition onto tetrasome to form nucleosome.

Fig S1a EM images of the reconstituted nucleosomes used for the study. Fig S2a SDS-PAGE analysis of the purified FACT and its individual subunits Fig S3a EM images of the reconstituted mono-tetrasomes

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