Omics · study · 2026
A maternal Polycomb imprint is maintained in early Drosophila development through lower-order methylation states
Listed in NCBI GEO
In an associated manuscript, we have measured by ChIP-seq the genome-wide distribution of total Histone H3, and Histone H3 di- and trimethylated at Lysine 27 (H3K27me2, H3K27me3) in early Drosophila embryos to measure establishment and maintenance of these modifications during the maternal-to-zygotic transition.
Description
We measured occupancy of these histone states at two timepoints corresponding to early embryonic cleavages (nuclear cycles 1-10, (NC1-10)) and late in the nuclear cycle following large-scale zygotic genome activation (nuclear cycle 14 (NC14)).
The experiment was performed in two batches, each with two biological replicates for a total of 4 replicates. Two of the replicates were performed on embryos expressing RpA70-EGFP, which facilitates staging of early embryos by nuclear density. The second set of replicates were performed on embryos collected from a cross between RpA70-EGFP mothers and OregonR fathers.
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This second batch of replicates allowed us to distinguish whether H3, H3K27me2, or H3K27me3 preferentially associates with maternal or paternal chromating through the mapping of single nucleotide polymorphisms present in either the maternal or paternal genotype. To define these SNPs, we also performed whole genome resequencing of both parental stocks. We find that H3K27me2/3 is asymmetrically present on maternally-derived chromatin at NC1-10, and that both marks equalize by late NC14.
In the associated manuscript, evidence is provided that the H3K27me2 mark is maintained over cleavage divisions, and that H3K27me3 is passively diluted over this time.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE327nnn/GSE327938 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE327938 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1453044 ncbi.nlm.nih.gov/bioproject/PRJNA1453044 ↗
project · from NCBI GEO
Topics
- Stated by source
- Drosophila melanogaster · Genome binding/occupancy profiling by high throughput sequencing · Genome variation profiling by high throughput sequencing
- From keywords
- Life Sciences
- Inferred from text
- Genetics 73%
Provenance · 1 source records, 9 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE327938 | 15 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].anzsrc:group:3105 | enrichment · NCBI GEO | taxonomy-embedding@1.1.0 | title+keywords+description (73%) |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:genome-binding-occupancy-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[method].geo_series_type:genome-variation-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[organism].NCBITaxon:7227 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |