<p>Binding of V1WT_06 to WIV-1 RBD.</p>

Individual CDRH1, CDRH2, and CDRH3 views have been shown with contact residues on the RBD shown as sticks and numbered based on WIV-1. <b>B)</b> Sequence alignment of a panel of SARS-CoV-2 Variant RBDs. Contact residues of V1WT_06 mAb on the WIV-1-RBD are indicated with black outline.

The colouring scheme describes the percent identity (PID) between the sequences as implemented in Jalview 2.11.4.0. <b>C)</b> Superimposed 3D structure of S2H97 (lime green), another RBD site V neutralising antibody (from PDB: 7M7W) and ACE2 (orange, from PDB: 8WLZ) shown around WIV-1 RBD – V1WT_06 (dark blue) Fab complex. Angle of approach of V1WT_06 in relation to S2H97 contrasts the RBM proximal, heavy chain driven interaction of V1WT_06 as opposed to RBM distal angle of approach of S2H97 [<a href="plosbiology.org/article/info:doi/10.1371/journal.pbio.3003882#pbio.3003882.ref017" target="_blank">17</a>]. <b>D)</b> and <b>E)</b> Neutralisation potency of Site V mAb S2H97 compared to V1WT_06.