Data · dataset · 2026
Investigating the molecular basis of polyurethane degradation in microbial communities
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<p dir="ltr">Over twenty-three million tons of polyurethane is produced annually, of which less than twelve percent is recycled.
Description
Most polyurethanes can only be recycled into equivalent-value products by depolymerisation under harsh conditions. The microbial depolymerisation of polyurethane, which can occur at ambient temperature, might provide a sustainable alternative to chemical recycling.
Here, we pursued two complementary aims towards the development of microbial systems for polyurethane recycling. First, we aimed to recombinantly express the <i>Pseudomonas protegens</i> polyurethane esterase PueA in novel hosts to enhance enzyme production. And secondly, we aimed to determine the molecular basis of a co-operative polyurethane degrading community of <i>P. protegens</i> and <i>Serratia marcescens</i>.
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Towards our first aim, we developed a plasmid, pus250_pueA, for recombinant expression of <i>pueA</i>. We then transformed pus250_pueA into six bacterial hosts and identified two, <i>Pseudomonas putida</i> 17484 and <i>S. marcescens</i>, with superior polyurethane clearance activity to the natural clearer <i>P. protegens</i>. For our second aim, we determined the growth dynamics, carbon preference, and transcriptional profile of our co-culture.
From our data, we hypothesise that <i>P. protegens</i> consumes adipate from polyurethane and preys upon <i>S. marcescens</i> for access to additional nutrients. Together these results will advance the development of sustainable microbially-driven methods for polyurethane recycling.</p>
Links
Where it is published
- DOI doi.org/10.25949/31846360.v1 ↗
DOI / persistent id · from zivahub uct ac za
Catalogue records · 1
- OAI-PMH record api.figshare.com/v2/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=oai%3Af… ↗
metadata API · from zivahub uct ac za
Topics
- From keywords
- Earth & Environmental Science · Life Sciences · Synthetic biology
Provenance · 1 source records, 8 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| ZivaHub | oai:figshare.com:article/31846360 | 13 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| concepts[field].anzsrc:field:310113 | mapping · zivahub uct ac za | vocabulary-mapper@1.0.0 | keywords['Synthetic biology'] |
| concepts[field].local:field:earth-environmental | mapping · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| description | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | /metadata/dc/description |
| license_text | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| publication_date | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| title | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | /metadata/dc/title |