<p>Antigenicity of bat sarbecoviruses using monoclonal antibodies isolated from SARS-CoV-2 breakthrough infections.</p>

For K_D, no bar indicates no binding by SPR. For EC₅₀, lower limit of detection: <0.08 µg/ml, upper limit of detection: >5 µg/ml. <b>(B)</b> Neutralisation (IC₅₀) titres shown for each mAb against bat sarbecovirus pseudotyped viruses (µg/ml), with limits of detection indicated (lower limit: <0.08 µg/ml; upper limit: >50 µg/ml for IC₅₀).

HEK293T cells stably expressing human ACE2 were used for all neutralisation assays. apart from RhGB07 and Rc-o319, where HEK293T cells overexpressing <i>Rh. alcyone</i> or <i>Rh. cornutus</i> were used, respectively. Scale bars (right) represent direction of increased binding or neutralisation. <b>(C)</b> RhGB07 trimer and RBD, with antibody competition groups highlighted, and are coloured with the same colouring as the SARS-CoV-2 trimer in (<a href="plosbiology.org/article/info:doi/10.1371/journal.pbio.3003944#pbio.3003944.g006" target="_blank">Fig 6B</a>).